UV Spectrum
UV Spectrum
Ultraviolet (UV) spectrum is what many people refer to as ultraviolet (UV) light. It consists of a range of wavelengths that are shorter than visible light with higher frequencies. The UV spectrum is merely one small part of the entire electromagnetic (EM) spectrum. As well, it is part of the EM spectrum that is not visible to the humans.
Subsequently, the UV spectrum has a range of wavelengths of around 10 nanometers (nm) to 400 nm. In addition, within the UV spectrum there are various subsections such as extreme UV (UVC) that is farthest from violet light and near UV (UVA) that is closest. Mainly, a lower wavelength has a higher frequency of UV light such as the extreme UV which contains between 121 nm and 10nm, near UV contains wavelengths of 315 nm.
UV Spectroscopy & The UV Spectrum
UV spectroscopy is a method utilized in scientific laboratories to define which wavelengths of light a chemical solution absorbs. This information makes it possible for researchers to analyze the contents of sample solutions. Additionally, absorption can be measured at a single wavelength to an expanded spectral range. UV spectroscopy supports the classification of reflectivity, absorption and transmission of various materials like pigments and filters.
Since the ultraviolet and visible (UV-Vis) range for the human eye is around 400 to 750 nm, specialized devices are needed to view UV spectrum below 400 nm. Likewise, UV spectroscopy is implemented with distinctive devices known as an ultraviolet-visible (UV-Vis) spectrophotometer as well as UV spectrometers.
UV spectrometers and UV-Vis spectrophotometers are common devices used in various fields such as applied research, life sciences, study of pigments, pharmaceuticals, chemistry, food research and quality control. Furthermore, the UV-Vis working range of the conventional UV-Vis spectrophotometer is 190 to 900 nm. In addition, there are also various spectral ranges that can vary depending on the device. Some UV-Vis spectrophotometers are designed for short-wavelength with absorption of UV wavelengths less than 180 nm. Likewise, these short-wavelength devices can be equipped to work beyond 175 nm by using an appropriate UV light source. Devices on the high-end expand the assessable spectral range into the NIR region to 3300 nm.
Ideally, a UV spectrometer or UV-Vis spectrophotometer has full-spectrum capabilities which provide innovation, efficiency and cost effective analysis. However, there are industries that use several different devices when characterizing UV and Vis spectrum.
UV spectroscopy devices such as the UV-Vis spectrophotometer are widely used in laboratories for several types of analyses like organic compounds, biological macromolecules and transition metal irons. Because different compounds are analyzed, various cuvettes or clear tubes are also needed. Cuvettes contain sample solutions for analysis and are composed of fine quartz so that UV light is not absorbed. High quality quartz is preferred in order to obtain optimum quality samples.
Further Resources on the UV Spectrum:
http://en.wikipedia.org/wiki/Ultraviolet
Conclusively, UV spectroscopy is an important technique that is vital for the progress of laboratory analysis in medicine, biology, chemistry, astronomy and an array of other fields. Moreover, utilizing a UV spectrometers or UV-Vis spectrophotometer allows UV spectrum to be analyzed with immense precision and expands the understanding of different properties.
Categories: UV Spectroscopy, UV Spectrum Tags: uv spectroscopy, UV Spectrum
What is UV Spectroscopy?
What is UV Spectroscopy?
UV Spectroscopy is the branch of physical science which deals with the study of the interaction between radiant energy and molecular particles of an object within the ultraviolet range. This study is based on the principle of dispersion of visible light through a prism corresponding to the wavelength and frequency. Simply, UV Spectroscopy is a method which is used to measure the dispersion of electromagnetic energy emission by molecules or atomic particles of an object.
Depending upon the intensity of light energy, molecules and atomic particles of materials are excited which causes an emission of electromagnetic particles. In other words, for the striking of ultraviolet ray, materials absorbed some energy which excites the electrons. Those excited electrons increase molecular vibration and shifts the electrons into a higher energy orbital. When the electrons are too excited, these emit in the form of electromagnetic particles. UV Spectroscopy relates this electromagnetic emission with the ultraviolet light intensity for further computation.
An electronic device, universally known as UV Spectrophotometer used this method for measuring radiation, absorption, abundance, composition, concentration and other significant properties of materials. As the atomic particles or molecules of a material are bounced off when ultraviolet light strike on it, so a UV Spectrophotometer can measure those material properties with maintaining a high degree of accuracy.
UV Spectroscopy Spectrum
The term ‘Spectrum’ is used to evaluate the data obtained by the UV Spectroscopy process which is nothing but a graphical representation of material properties developed by plotting material properties as a function of frequency or wavelength. Later analyzing the Spectrum, color, concentration and other significant properties are determined.
To perform UV Spectroscopy, a light beam is divided into two parts, one is the sample light beam and other is the reference light beam. The sample light beam is then passed through a target sample. As well, the reference light beam is also passed through a controlled solvent which does not contain the target sample. A UV Spectrophotometer receives both light beams after they passed completely through the predetermined path. Then it compares the transmission difference of the reference and sample light beams. This procedure is simple and so accurate that there is no other alternative process by which the mentioned properties can be measured more accurately and effectively.
Importance of UV Spectroscopy
The importance of using UV Spectroscopy is beggar description. It is not only used in laboratories for scientific analysis but also used in every modern industry in order to ensure quality production. Even in biological analysis, bio-medical engineering and space technology field it has huge applications. Different types of UV Spectrophotometer and UV Spectrometer is made based on UV Spectroscopy. Hopefully through this article many of your questions must have been answered concerning what UV Spectroscopy is. In closing if one wants to ensure quality production in their industry, there may be no alternate choice besides using UV Spectroscopy.
Please feel free to contact us if you have any questions regarding UV Spectroscopy.
Categories: UV Spectrophotometer, UV Spectroscopy, UV Visible Spectroscopy Tags: uv spectrophotometer, uv spectroscopy, uv visible
UV Visible Spectroscopy
UV Visible Spectroscopy
UV visible spectroscopy refers to the study of reflectance spectroscopy or absorption spectroscopy within a certain 
spectral region. To execute a test, one uses a light beam from the visible range to near the ultraviolet and infrared range. As the color, concentration and other significant properties of materials and chemicals are directly affected by the reflectance or absorption of light energy, so making this criterion as base electromagnetic spectrum is evaluated by a UV Spectrophotometer. This UV Spectrophotometer is nothing but a piece of electronic equipment which employs the UV visible spectroscopy principle.
To perform UV visible spectroscopy, a UV Spectrophotometer uses two separate light sources, one for producing ultraviolet light and another for producing visible light. A deuterium lamp supplies ultraviolet light beam and a tungsten lamp supplies a visible light beam during the testing period. The light beam is guided to pass through a diffraction grating which contains slits and hits. Only monochromatic light which means single wavelength light beam can pass through a slit of grating. Any unwanted scattered light beam is removed by grating filters. Different monochromatic light can also be passed by rotating diffraction grating to give a setup corresponding to their wavelength.
The monochromatic light beam is then guided to strike on a split mirror which divides the light beams into two separate beams of which one is used as sample light beam and another is reference light beam. The sample light beam is passed through a target sample and reference light beam is passed through a controlled solvent. A UV Spectrophotometer receives both light beams once after they passed the predetermined path. Then it compares the transmission difference of the reference and sample light beams for the computation of electromagnetic spectrum.
Demand For UV Visible Spectroscopy
UV visible spectroscopy has great demand on biological research and analytical chemistry for the identification of unfamiliar samples, such as highly conjugated metallic and organic compounds, macromolecules of biological species, transition metals, etc. These identifications are usually carried in a solution. Now-a-days it is also used in space technology for the identification of new objects and in the industry for ensuring quality production. Color, concentration, absorbance capacity, temperature, frequency, wavelength, abundance, etc. are significant properties of a solution or material and can also be determined by this method.
UV visible spectroscopy is an unconventional process, so no direct contact with the sample object is required during the testing period. Also, the testing can be done in an UN-contaminating way. That’s why UV Spectrophotometer has made a strong place from the scientific field to industrial field in order to serve different purposes.
Questions About UV Visible Spectroscopy?
Hopefully through this article many of your questions must have been answered regarding UV visible spectroscopy and UV Spectrophotometers. So, if one has to do such testing in a specific field, it is advisable to purchase a UV Spectrophotometer. Since there are no other devices or systems that can be used to serve those purposes effectively and efficiently with a high degree of accuracy. Also a UV Spectrophotometer is very easy to operate and no experienced personnel are required to operate it. If you have any questions on uv visible spectroscopy please contact us.
UV Spectrophotometer Introduction
UV Spectrophotometer
A UV Spectrophotometer is a piece of equipment which is used to determine the radiation intensity of light so that concentration, color and other significant information of a product or object can be evaluated. It is basically an electronic device which working function is based on the principle of photo spectrometry by relating the photon characteristic with the wavelength of light. It can measure the intensity of normal light as well as the intensity of infrared radiation. Different electromagnetic spectrum and electric transitions can also be identified by a UV Spectrophotometer.
Reflectance measurement, linear energy absorption range and spectral bandwidth are the key features of a Spectrophotometer. In order to determine the radiation intensity, UV Spectrophotometer sends a beam of light through a test piece and measure the absorption energy of the test piece. From the sum of the absorption energy, intensity of radiation is calculated. Ultimately concentration, color and other significant criteria of the test piece is computed. That’s why UV Spectrophotometer is not only used in the laboratory or scientific research but also widely used in different industry to estimate the characteristics of their manufactured products.
Different Types of UV Spectrophotometer
Though there are a variety of UV Spectrophotometer are present in the market, but most of the people appreciate three different types of UV Spectrophotometer which are UV single beamed, UV double beamed and UV split beamed Spectrophotometer. These UV Spectrophotometer can identify the color properties of an object which radiation intensity range is almost 400 to 700 nm. At high radiation these can also measure fluorescence radiation spectrum. This spectrum is very important for the accurate measuring of color properties of many objects.
By estimating the relative radiation intensity of the light beam before and after the test piece is inserted, a UV single beamed Spectrophotometer measure the properties of the object. Whereas a UV double beamed Spectrophotometer measure the properties of the object by comparing the radiation intensity of two light beam paths. One path is for test piece and another for reference sample. Though UV single beamed Spectrophotometer has a large dynamic range but UV double beamed Spectrophotometer is more preferable due to ease in operation and more stability.
Formats for A UV Spectrophotometer
D/8 and O/45 are the different two formats of UV Spectrophotometer. For the analysis of spherical circumstance D/8 format is used whereas O/45 is used for the analysis of linear circumstance. Both formats are widely used in order to recognize light sources as well as various types of environmental objects. And this is done by analyzing the observed photo effects.
There is no doubt this photometer is really essential in the field of modern industry. The importance of the use of UV Spectrophotometer can be easily highlighted by giving an example of ink industry. Only a UV Spectrophotometer can ensure the correct color composition of ink. Users must make the correct decision, whether they have needed a UV Spectrophotometer or not. Based on the information above users will have a much easier time selecting a uv spectrophotometer to fit ones needs.
